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9982-1013341
9982-1013458
Exper iment s
E l ec t rophores i s
DNA Fingerprint
DNA fingerprinting is an important method used in molecular genetics. It
is now inconceivable, for instance, for criminal forensics to do without it.
Other examples of areas where it can be applied are paternity tests, the
analysis of genetic diseases, and identifying victims after natural catastro-
phes or accidents. During this procedure, DNA fragments are generated by
way of a polymerase chain reaction (PCR), and then separated using gel
electrophoresis. In our kit, however, the DNA fragments have already been
separated so that the pupils only have to carry out electrophoresis. Using
the DNA profiles thus obtained, your pupils can draw their conclusions
about where they come from (scene of the crime, victim, or suspect). While
doing this, the pupils learn about the practicalities of using molecular ge-
netics techniques, and will be able to discuss the DNA profiles in detail
once they have completed the experiment. This topic is also a wonderful
way of introducing a discussion about other potential applications for DNA
finger printing, as well as about associated legal and ethical issues.
Scope of delivery:
DNA from a victim, 120 µl, DNA from the scene of the crime, 120 µl, DNA
from suspect n° 1, 120 µl, DNA from suspect n° 2, 120 µl, electrophoresis
buffer, 50x conc., 50 ml, agarose, 6 g, DNA staining solution, 200x conc.,
1.5 ml
Packaging size: Enough for 10 experiments
&
D/E/F/S/I/P
9982-1013458
Additionally required:
Electrophoresis chamber (9982-1012852, page 114), power supply (9982-
1010263, page 116) and micropipette (9982-1013416, page 111)
Supplies for Electrophoresis Experiments:
Agarose
for DNA gel electrophoresis, compatible with usual electrophoresis buffers
and DNA staining methods, 50 g.
9982-1013341
Methyl Blue Staining Concentrate
(not shown)
Concentrated 200 times (2x 1.5 ml), staining in approx. 15 – 20 min. with a
high level of sensitivity. Sufficient for approx. 20 stainings of medium-sized
gels.
9982-1013342
TAE Electrophoresis Buffer
(not shown)
50ml, electrophoresis buffer concentrated 50 times, contains 2M Tris,
1M ascetic acid, 50mM EDTA, pH 8.5.
9982-1013343
Gel Loading Buffer
(not shown)
Concentrated 6 times – 2x 1.5 ml (with glycerine and bromophe-
nol blue as colour marker).
9982-1013347
100 bp DNA Ladder
(not shown)
250μl, ready for use, dissolved in 1x gel loading buffer,
with bromophenol blue as a colour marker. Size range
of the fragments 100 – 1,000 bp. Sufficient for approx.
25 agarose gels.
9982-1013348
ATTENTION:
This experiment requires an
electrophoresis chamber and
a suitable power supply.
(Page 114)
