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9982-1005935
9982-1005937
3 B S c i e n t i f i c ® B i o l o g y
Exper iment s
E l ec t rophores i s
Diagnosing Gene Defects
Examine the genetic mutation responsible for sickle cell anaemia. Your
students will use agarose gel electrophoresis to study sickle cell anaemia –
a painful and ultimately fatal condition resulting from a genetic mutation
which alters the body’s haemoglobin. They’ll search for changes in a non-
human DNA sample to diagnose sickle cell anaemia. In the process, they’ll
learn about genes and how genetic mutations can cause disease.
Scope of delivery:
4 DNA samples (mother’s DNA, father’s DNA, daughter’s DNA, unborn
child’s DNA), each 150 ml, 2 TBE Buffer concentrate (125 ml) 10X, 1 melt
and bast agarose gel (400 ml) 0.8%, 1 Neo/BLUE DNA stain concentrate
(100 ml) 10X, 1 staining tray, description.
30x22x10 cm, 1.5 kg
&
D/E
9982-1005937
Simulating DNA Paternity Testing
Use agarose gel electrophoresis to test family ties. Simulate how DNA fin-
gerprinting can be used to identify the genetic relationship between child
and an alleged father. Your class will use the results of an electrophoresis
of non-human DNA, their knowledge of human inheritance and their scien-
tific problem solving skills to solve a scenario-based paternity case and de-
termine the identify of a child’s father.
Scope of delivery:
3 DNA samples (mother’s DNA, alleged father’s DNA, child’s DNA) each
150 ml, 2 TBE buffer concentrate (125 ml) 10X, 1 melt and cast agarose gel
(400 ml) 0.8%, 1 Neo/BLUE DNA stain concentrate (100 ml) 10X, 1 staining
tray, description.
30x22x10 cm; 1.5 kg
&
D/E/S
9982-1005939
Genetic Diagnosis of Cancer
Your students will learn how revolutionary breakthroughs in gene technol-
ogy can be used to not only detect cancer but predict its occurrence based
upon hereditary traits. They’ll use the electrophoresis results of non-hu-
man DNA samples to simulate this procedure. In the process, they’ll detect
a specific cancer and study the hereditary tendencies of the condition.
Scope of delivery:
DNA samples (Mary’s DNA, Samantha’s DNA, Fran’s DNA, normal control
DNA) each 150 ml, 2 TBE Buffer concentrates (125 ml) 10X, 1 melt and cast
agarose gel 0.8% (400 ml, 1 Neo/BLUE DNA stain concentrate (100 ml)
10X,1 staining tray, description.
30x22x10 cm; 1.5 kg
&
D/E
9982-1005935
ATTENTION:
This experiment requires an
electrophoresis chamber and
a suitable power supply.
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